FACS analysis of lymphoid cultures generated from CD34⁺CD38⁻lin⁻CD7⁺cells.

Three hundred cells were cultured in B-lymphoid conditions for 20 days, then switched to NK conditions (in IL-15) and cultured for another 14 days. (A) Total culture showing R1 gate. (B-E) Analysis of cells from R1 gate. Size (FSC) and side scatter (SSC) of (F) CD19⁺CD56⁻ B cells, (G) CD19⁻CD56⁺ NK cells, and (H) CD19⁻CD1a⁺ dendritic cells gated from quadrants shown in (D) and (E).