SDS-PAGE of fibrinogenolysis fragments digested in the presence of 1 mM EDTA or 1 mM Ca⁺⁺.

Normal fibrinogen (N), variant fibrinogens Milano XII (M) and St Gallen I (S). Lane S illustrates the size of the intermediate plasmin degradation fragment D2 (see “Results”). (A) Nonreduced samples. (B) Reduced samples. W, molecular weight standard. Fibrinogen (0.4 mg/mL) was digested with plasminogen (10 μg/mL) and streptokinase (40 U/mL) for 4 hours at 37°C.