Immunoblots of unreduced normal fibrinogen and of fibrinogen Milano XII.

(A) Detection with anti–human fibrinogen antibodies. (B) Detection with anti–human serum albumin antibodies. N, normal fibrinogen; M, fibrinogen Milano XII; HMW, high-molecular-weight fibrinogen (340 kd); LMW, low-molecular-weight fibrinogen (305 kd); LMW′, low-molecular-weight fibrinogen (270 kd). Amount of fibrinogen loaded: panel A, 0.44 μg; panel B, 2.4 μg. Dotted lines connect the albumin-linked bands in panel B with their counterparts in panel A.