FISH analysis of the t(5;10)(q33;q22) in primary patient cells.

(A) FISH analysis using PDGFβR- andH4-locus–specific probes. CosB, a PDGFβR-specific genomic probe, gave 3 hybridization signals on normal 5 and on der(5) and der(10) originating from t(5;10), respectively. Both YACs 781F5 and 876H2, for 10q21.3 to q22.1, hybridized with the normal chromosome 10 and with the der(5). No signals were present on der(10). (B) Dual-color FISH with CosB (red) and PAC29F6 (green) showed the presence of a fusion signal on der(5), whereas a green signal (PAC29F6) was present on the normal 10, and a red signal was seen (CosB) on the normal 5 and the der(10).