Spontaneous and anti-CD3–induced apoptosis in PBMCs from HIV-infected children.

PBMCs of HIV-infected children were cultured in medium for 3 days without anti-CD3 mAb to determine spontaneous lymphocyte apoptosis and with anti-CD3 mAb (0.5 μg/mL) for 48 hours to determine activation-induced lymphocyte apoptosis. Data shown represents percentage of lymphocyte apoptosis, estimated by propidium iodide staining. Symbols represent individual patients who were on non-PI– (left) or on PI- (right) containing antiretroviral therapy regimens. Mean value in each data set is represented by a solid line.