American Society of Hematology

Fig. 5.

$Fig. 5. dose response of dipyridamole inhibition of sickling-induced Na+ and Ca++ influx. / Washed cells were suspended at 10% hematocrit in the nominal dipyridamole concentrations indicated and then divided for separate measurements of Na+ (●) and K+ (▪) fluxes (A) and Ca++ influx (▴) (B). For both measurements, deoxygenation was carried out under similar conditions in parallel. Net Na+ and K+ fluxes were measured in the presence of 100 μM ouabain. Sickling-induced fluxes were calculated as described in Figure 2 and Figure 4. The fraction of the flux remaining relative to control samples without dipyridamole was calculated for each of 3 experiments.$

dose response of dipyridamole inhibition of sickling-induced Na⁺ and Ca⁺⁺ influx.

Washed cells were suspended at 10% hematocrit in the nominal dipyridamole concentrations indicated and then divided for separate measurements of Na⁺ (●) and K⁺ (▪) fluxes (A) and Ca⁺⁺ influx (▴) (B). For both measurements, deoxygenation was carried out under similar conditions in parallel. Net Na⁺ and K⁺ fluxes were measured in the presence of 100 μM ouabain. Sickling-induced fluxes were calculated as described in Figure 2 and Figure 4. The fraction of the flux remaining relative to control samples without dipyridamole was calculated for each of 3 experiments.

This Feature Is Available To Subscribers Only