American Society of Hematology

Fig. 5.

$Fig. 5. NK cell and mast cell production from CD34+CD81+, CD34lowCD81+, and CD34+CD81high cells cultured on stroma derived from NOD/SCID fetal-mouse liver. / (A) Appearance of NK and mast cells derived from the CD34+CD81high fraction in 4-week culture with NOD/SCID fetal-mouse liver stroma. The photograph shows 2 mast cells and a large granular NK cell stained with May-Grünwald–Giemsa solution (× 1000). (B) The presence of mast cells were confirmed by the reaction with a mAb specific to human tryptase on APAAP staining. The photograph shows positive staining for tryptase in mast cells derived from the CD34+CD81high fraction (× 1000). (C) Flow cytometric profiles of expression of CD56 and CD3 on cells cultured from CD34+CD81+, CD34lowCD81+, and CD34+CD81high cells on NOD/SCID fetal-mouse liver stroma for 4 weeks.$

NK cell and mast cell production from CD34⁺CD81⁺, CD34^lowCD81⁺, and CD34⁺CD81^high cells cultured on stroma derived from NOD/SCID fetal-mouse liver.

(A) Appearance of NK and mast cells derived from the CD34⁺CD81^high fraction in 4-week culture with NOD/SCID fetal-mouse liver stroma. The photograph shows 2 mast cells and a large granular NK cell stained with May-Grünwald–Giemsa solution (× 1000). (B) The presence of mast cells were confirmed by the reaction with a mAb specific to human tryptase on APAAP staining. The photograph shows positive staining for tryptase in mast cells derived from the CD34⁺CD81^high fraction (× 1000). (C) Flow cytometric profiles of expression of CD56 and CD3 on cells cultured from CD34⁺CD81⁺, CD34^lowCD81⁺, and CD34⁺CD81^high cells on NOD/SCID fetal-mouse liver stroma for 4 weeks.

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