Phosphorylated CD84 and Ly-9 associate with SAP at the plasma membrane.

To verify the results obtained with the yeast 2-hybrid system and immunoprecipitation in COS-7 cells, a fluorescence microscopy approach was used. COS-7 cells were transfected with SAP and Fyn along with CD84 or Ly-9. The cells were then stained for SAP and Ly-9 or CD84. Colocalization of SAP and Ly-9 or CD84 at the cell membrane can be visualized by yellow fluorescence where the 2 fluorochromes colocalize.