CD28 activation of RE/AP is resistant to FK506 in B cells.

Transfections and stimulations were performed as in Figure 1, with the expression and reporter constructs as denoted in the figure. For RE/AP and CD8/28 cotransfections, the results were standardized to the activation of PMA/OKT8 (n = 100); the actual average luciferase counts were 6613 for Bal17 and 73 938 for DT40. For NFAT transfections, the results were standardized to the activation of PMA/ionomycin; the actual average luciferase counts were 2297 for Bal17 and 70 289 for DT40. FK506 was added to some samples at a final concentration of 100 ng/mL before stimulation. For samples transfected with the NFAT reporter, ionomycin was added at a final concentration of 1 μM. The results shown are the average of 3 independent transfections. Error bars reflect the SD from the mean.