Platelet glycoprotein mRNA studies.

Semiquantitative RT-PCR analysis of the genes for β-actin, GPIbβ, GPIX, and Gsα (GNAS1) using platelet RNA of affected patients (V:7, V:8, and V:3), obligate carrier (IV:1), and controls (Crl). Starting from the same amount of platelet RNA, patients and controls have comparable levels of β-actin expression. All patients have a very weak expression of the GATA1-regulated genesGPIbβ and GPIX, only showing a visible fragment after performing a nested PCR. Patients also show a weaker signal for the nondirectly GATA1-regulated gene GNAS1.