Identification of transplanted donor cells in chimeric mice using FISH.

To trace the transplanted donor cells, spleen and kidney sections from chimeric mice with or without nephritis were subjected to FISH using a Y painting probe (4 per group). Representative photographs are shown. (A) Spleen of control female mouse; (B) spleen of control male mouse; (C) spleen of untreated chimeric mouse; (D) spleen of chimeric mouse treated with anti-GBM serum (day 3); (E) kidney of control female mouse; (F) kidney of control male mouse; (G) kidney of untreated chimeric mouse; and (H) kidney of chimeric mouse treated with anti-GBM serum (day 3). Red dots show Y antigen–positive donor cells.