![Fig. 2. Bone marrow reconstitution using a retrovirus. / Bone marrow from female recipient mice was reconstituted with genetically engineered male donor cells using a transplantation-based method. Eight weeks later, DNA was extracted from the bone marrow, spleen, and liver of these mice and subjected to PCR for mouse Y antigen (395 base pairs [bp]). A representative photograph from 9 chimeric mice is shown (A). DNA from spleen was also subjected to semiquantitative PCR for the human IL-1Ra gene. This pair of primers may yield both cDNA (263 bp) and gDNA (1045 bp).24 As a standard, different amounts of IL-1Ra cDNA, corresponding to 1, 0.5, 0.2, 0.1, and 0.05 copies per cell, were used. A representative photograph from 4 individual mice is shown. M: DNA marker (φ × 174/HaeIII digest) (B).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/1/10.1182_blood.v98.1.57/6/h81311232002.jpeg?Expires=1724155227&Signature=u03bHCb92xzoN-80ZfrafaL-N-potOZzzk7SSp-ONG3q-vnEsluModeoA1Af6OMCB0f5SMa2wSiKlxgV-EO387b0CyCSQHdoFH33NcCTD~3aZT9cUiYPL30ma-0f~eflr0G3u~VOEWaVW97vJdmcM~MyprRMGFOFn4-qSi8MhzxpGxtwy2I0vYDCy--b61S6N51-RGjTBQTl6FQnWm0GfKRKODu1B~t2Ccd92qYCLUbXqg0~OVVZ3aGdndtw0o8Owk3upaB3Quua4fPA1ZOyQx3dWuAqUdJXFNMiXUaPevnQx4tb9q0zowqiuN171hyj6qrU83KM-6BKiwsIF75JzA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Bone marrow reconstitution using a retrovirus.
Bone marrow from female recipient mice was reconstituted with genetically engineered male donor cells using a transplantation-based method. Eight weeks later, DNA was extracted from the bone marrow, spleen, and liver of these mice and subjected to PCR for mouse Y antigen (395 base pairs [bp]). A representative photograph from 9 chimeric mice is shown (A). DNA from spleen was also subjected to semiquantitative PCR for the human IL-1Ra gene. This pair of primers may yield both cDNA (263 bp) and gDNA (1045 bp).24 As a standard, different amounts of IL-1Ra cDNA, corresponding to 1, 0.5, 0.2, 0.1, and 0.05 copies per cell, were used. A representative photograph from 4 individual mice is shown. M: DNA marker (φ × 174/HaeIII digest) (B).
