Tyrosine nitration in thymocytes.

(A) Western blot analysis of thymocyte extracts with an antinitrotyrosine antibody showed that SIN-1 induced a high level of tyrosine nitration on several proteins, an effect partly reversed by exogenous SOD. These data are representative of 3 different experiments. (B) Western blot analysis of normal thymic extracts with an antinitrotyrosine antibody showed 2 major bands in all the samples studied. The data shown are representative of 3 different experiments and 10 distinct thymic samples.