Fig. 10.
Fig. 10. Immunoprecipitation and isoforms of cell surface human Ly-9 protein. / (A) Detergent lysates of surface-labeled Daudi cell line, 300.19 cells transfected with full-length human Ly-9 cDNA, Ly-9 cDNA lacking the fourth immunoglobulinlike domain (HLy-9-Δ4), and 300.19 untransfected cells were immunoprecipitated with the HLy-9.1.25 mAb and analyzed under reducing and nonreducing conditions on a 10% sodium dodecyl sulfate–polyacrylamide gel. Molecular weights (kd) were determined by the migration of a known protein standard. (B) cDNA was made with mRNA from 300.19 cells transfected with full-length human Ly-9 cDNA, Ly-9 cDNA lacking the fourth immunoglobulinlike domain (HLy-9-Δ4), and B-cell line Daudi. Ethidium bromide staining of the PCR products after gel analysis. A molecular weight ladder is shown as an estimate of DNA band size.

Immunoprecipitation and isoforms of cell surface human Ly-9 protein.

(A) Detergent lysates of surface-labeled Daudi cell line, 300.19 cells transfected with full-length human Ly-9 cDNA, Ly-9 cDNA lacking the fourth immunoglobulinlike domain (HLy-9-Δ4), and 300.19 untransfected cells were immunoprecipitated with the HLy-9.1.25 mAb and analyzed under reducing and nonreducing conditions on a 10% sodium dodecyl sulfate–polyacrylamide gel. Molecular weights (kd) were determined by the migration of a known protein standard. (B) cDNA was made with mRNA from 300.19 cells transfected with full-length human Ly-9 cDNA, Ly-9 cDNA lacking the fourth immunoglobulinlike domain (HLy-9-Δ4), and B-cell line Daudi. Ethidium bromide staining of the PCR products after gel analysis. A molecular weight ladder is shown as an estimate of DNA band size.

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