Flow cytometric analysis of human peripheral blood leukocytes and bone marrow cells staining with anti–human Ly-9 mAb.

These results are representative of those obtained in at least 6 experiments for blood samples and 3 for the bone marrow cells. PBL indicates peripheral blood leukocyte. (A) Peripheral blood mononuclear cells (PBMCs) were incubated with biotinylated HLy-9.1.84 mAb and CD19-FITC, CD3-APC, CD4-FITC, or CD8-perCP washed and incubated with avidin PE. (B) PBMCs were incubated with biotinylated HLy-9.1.84 mAb and CD56-FITC and CD16-PE washed and incubated with avidin-Cy-Chrome. (C) Bone marrow cells were stained with biotinylated HLy-9.1.84 mAb and CD19-FITC washed and incubated with avidin-PE. Cells with forward and light scatter characteristics of lymphocytes were gated for experiments shown in panels A, B, and C. (D) Peripheral blood cells were stained with biotinylated HLy-9.1.84 mAb washed and incubated with avidin-PE. Cells with forward and light scatter characteristics of monocytes or granulocytes were gated. Dotted lines indicate staining of the negative control in histograms.