Overexpression of the intracellular domain of FcαRI inhibits the binding of IgA beads to Ba/F3 cells.

Ba/F3_FcαRI cells overexpressing gag-tagged FcαRI constructs (A) were checked for FcαRI expression by FACS flow cytometry (B). Cells were used for IgA-binding assays and were cytokine starved and stimulated with either buffer (■) or IL-3 (░) (C). After stimulation rosette assays were performed, and binding of IgA beads was counted. Results are expressed as rosette index (number of beads/100 cells) and as means ± SE (n = 3).