RT-PCR analysis of 5-lipoxygenase mRNA expression in primary hematopoietic cells and cell lines.

By RT-PCR, 5-lipoxygenase mRNA was expressed in PB and BM CD34⁺ progenitors, PB PMNCs, MNCs, and at a low level in the cell lines THP-1 and HL60. No specific PCR signal could be observed in the negative control cell line SK-N-LO, in BM stromal cells, and in KG1a cells. Similar RNA amounts were used in the reactions, as demonstrated by RT-PCR for β-actin expression.