Effect of DC maturation on tumor antigen presentation of mRNA-transfected Mo-DCs.

IFN-γ production by the CTL clone was measured after coculture with HLA-A2⁺ Mo-DCs electroporated with Melan-A mRNA. iMo-DCs, Mo-DCs transfected at the immature stage and used as such; Mo-DCa, Mo-DCs transfected at the mature stage after LPS+TNF-α stimulation; Mo-DCb, Mo-DCs transfected at the immature stage, matured by LPS+TNF-α and then assayed for Melan-A–specific CTL clone stimulation. Results are shown as mean ± SD (n = 4). * P < .05.