Fig. 6.
Engagement of PSGL-1 by a monoclonal antibody triggers the formation of Mac-1 clusters and F-actin patches in PMNL.
PMNLs were preincubated with the anti–PSGL-1 antibody PL2 (20 μg/mL) and then were washed and incubated for 3 minutes in the presence of rabbit anti–mouse IgG F(ab′)2 fragments (10 μg/mL) in standard conditions. Samples were stained with FITC-conjugated anti-CD11b antibody and rhodamine-phalloidin and processed for confocal laser scanning microscopy; A and B show F-actin and CD11b staining, respectively. In the overlay (C), yellow represents colocalization of the 2 stainings. The figure shows an aggregate of 2 cells. Results were reported from a representative of 3 experiments.
