Inhibition of JAK2 with AG490 decreases SDF-1α–induced migration of CTS cells or CD34⁺ human bone marrow progenitors.

(A) CTS cells were pretreated with various concentrations of AG490 or control DMSO (0) for 2 hours. The effect of AG490 on cell migration was analyzed in a migration assay in the absence of SDF-1α (■, medium control), in the presence of SDF-1α in both the upper and lower chambers (▪), or with SDF-1α in the lower chamber only (▨). (B) CD34⁺ human bone marrow cells were pretreated with various concentrations of AG490 or control DMSO (0) for 2 hours. Cell migration in response to medium alone (−) or SDF-1α (+) was measured. Migration is shown as the percentage of cell input. The data represent the mean ± SD of 3 separate experiments performed in triplicate. *P < .05 as compared with control. **P < .01 as compared with control.