SDF-1α induces the tyrosine phosphorylation of STAT.

Total cell lysates from untreated or SDF-1α–treated (20 nM) CTS cells were immunoprecipitated with anti-STAT2 (A) or anti-STAT4 (B) antibody and analyzed by Western blotting with PY99 antiphosphotyrosine antibody (pTyr). After stripping, the membrane was reprobed with anti-STAT2 antibody (A, lower panel) or anti-STAT4 antibody (B, lower panel).