Sensitivity for Rapa-induced apoptosis is acquired during mo-DC differentiation.

Monocytes were cultured with IL-4 and GM-CSF as described in “Materials and methods.” On different days, Rapa (10⁻⁷M) was added for 48 hours. After 48 hours of incubation with or without Rapa, cells were harvested and analyzed using Hoechst. Data shown are the mean (± SD) percentages of apoptosis obtained from duplicate cultures. The results are representative of 4 independent experiments. *P < .05, Student t test for paired samples.