Fig. 4.
Frequency estimate of 12B1-D1 cells surviving treatment with AP20187.
Limiting dilution was performed. (A) 12B1-D1 cells were plated in the presence of 40 nM AP20187. (B) The cells were previously incubated for 6 hours with 40 nM AP20187, washed, and then added to 96-well plates. (C) The cells were previously heat shocked at 42°C for 1 hour, then incubated for 6 hours with 40 nM AP20187, washed, and then seeded into 96-well plates. Individual wells were considered negative if there was no cell growth after 2 weeks. The 95% confidence intervals (CIs) of the frequencies and χ2 estimates of probability were calculated. P > .05 is statistically significant and confirms that the data follow “single hit” kinetics.
