IFN production of ex vivo T lymphocytes in response to allogeneic third-party DCs.

Purified spleen T lymphocytes were obtained from naive BALB/c (H-2^d, black triangles), FVB (H-2^q, open squares) mice, or BALB/c mice grafted with 10⁶ BM cells from FVB mice plus apoptotic SCs of C57BL/6 (H-2^b) mice (noted TP, black circles) at day 45 to day 50 post-BMT. These T lymphocytes were used as responders and C57BL/6 mature DC as stimulants in a one-way mixed leukocyte reaction. The IFN-γ production was measured 48 hours later in the supernatant of cultures by enzyme-linked immunosorbent assay. The grafted mice (noted TP) included in this analysis had a complete FVB phenotype at day 45 to day 50 post-BMT. Four individual mice were tested in each group. A bold bar indicates the mean for each group. P = .739.