Fig. 3.
Fig. 3. PGE2 inhibits the production of IL-12p70 and IL-12p40 in DCs stimulated with CD40L or LPS. / DCs (4 × 104 cells in 0.2 mL) were stimulated with CD40L-transfected J558 cells (5 × 104 cells in 0.2 mL) or with LPS (250 ng/mL), either in the absence or in the presence of PGE2 (10−6 M). The supernatants were harvested after 48 hours and analyzed for IL-12p40 (▥) and IL-12p70 (▪) contents with specific ELISAs. The data are shown as mean (±) of triplicate cultures and represent one experiment of 4 that all gave similar results.

PGE2 inhibits the production of IL-12p70 and IL-12p40 in DCs stimulated with CD40L or LPS.

DCs (4 × 104 cells in 0.2 mL) were stimulated with CD40L-transfected J558 cells (5 × 104 cells in 0.2 mL) or with LPS (250 ng/mL), either in the absence or in the presence of PGE2 (10−6 M). The supernatants were harvested after 48 hours and analyzed for IL-12p40 (▥) and IL-12p70 (▪) contents with specific ELISAs. The data are shown as mean (±) of triplicate cultures and represent one experiment of 4 that all gave similar results.

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