Fig. 2.
The combination of TNFα and PGE2 induces IL-12p40 gene expression but fails to induce IL-12p35.
DCs (3 × 105 cells in 2 mL) were stimulated for 6 hours with one of the following stimuli: TNFα (50ng/mL) and PGE2 (10−6 M) or LPS (250 ng/mL) in the presence of IFNγ (1000 U/mL), as indicated, and lyzed for mRNA extraction. The expression of p35, p40, and β2m was analyzed with RT-PCR (see “Materials and methods”). The data shown are from a representative experiment of 3 performed.
