Increased expression of CD100 by B and T cells of MRL/lpr mice.

Lymphocytes from the inguinal and popliteal lymph nodes of 8-week-old (8 wk) (left) or 16-week-old (16 wk) (right) MRL/lpr mice (thick lines) or MRL/n mice (thin lines) were stained with PE-conjugated anti-CD4 and FITC-conjugated anti-CD8 (A,B) or PE-conjugated Thy1.2 and FITC-conjugated anti-B220 (C), and biotinylated anti-CD100 mAb plus APC-conjugated steptavidin (A-C). CD4⁺ (A), CD8⁺ (B) or B220⁺Thy1.2⁻ (C) cells were gated and CD100 expression was analyzed by flow cytometer. The profiles of cells stained with APC-conjugated steptavidin alone are also shown (A-C, dashed line).