Fig. 5.
Fig. 5. Serum levels of sCD100 and titers of antibodies in mice immunized with a TI Ag. / C57BL/6 mice (8 weeks old) each received an intraperitoneal injection of 50 μg TNP-LPS in PBS (n = 5) at day 0. Serum from individual mice was collected at indicated times. Concentrations of sCD100 (A) and serum titers of anti-TNP IgG1 antibodies (B) were determined by ELISA. The concentrations of anti-TNP IgG1 antibodies were established by comparison to standard curves created from pooled sera of C57BL/6 mice 14 days after immunization. The concentration of anti-TNP antibodies present in a 1:1000 dilution of pooled immune sera of C57BL/6 mice was defined as one arbitrary unit.18

Serum levels of sCD100 and titers of antibodies in mice immunized with a TI Ag.

C57BL/6 mice (8 weeks old) each received an intraperitoneal injection of 50 μg TNP-LPS in PBS (n = 5) at day 0. Serum from individual mice was collected at indicated times. Concentrations of sCD100 (A) and serum titers of anti-TNP IgG1 antibodies (B) were determined by ELISA. The concentrations of anti-TNP IgG1 antibodies were established by comparison to standard curves created from pooled sera of C57BL/6 mice 14 days after immunization. The concentration of anti-TNP antibodies present in a 1:1000 dilution of pooled immune sera of C57BL/6 mice was defined as one arbitrary unit.18 

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