Fig. 1.
![Fig. 1. Release of sCD100 from the surface of 2B4 cells stimulated with ConA. / 2B4 cells (1 × 107 cells/lane) were surface-labeled with biotin and then cultured in the presence or absence of ConA (4 μg/mL) for the time indicated, at 37°C. Cell lysates (1% NP-40) or culture supernatants (sup) were immunoprecipitated with BMA-12. Immunoprecipitates were subjected to SDS-PAGE in reducing (2ME [+]) or nonreducing (2ME [−]) conditions, electrophoretically transferred to nitrocellulose membranes, and blotted with streptavidin-POD. Molecular weight markers (Mr) are indicated on the right.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/11/10.1182_blood.v97.11.3498/6/h81111099001.jpeg?Expires=1726821643&Signature=1danVolyt8DYHlsCixZEtBtCx5SRjHtMNo0DHSexk8qKsAZOJFXRlJ5M0faD18GKF-QSZCQDraTI6c3TgXIEgCVbMjS82ZYAOj-bAvlpgbsoX~Jb~HLeMbH7pN5owOgNmJl3IEif~uKxEjLf5kMbKymH61-2ig4R8WhentB6I7rLUhiNyannGqlrJaJP2By~9zqhRIER1tPQr4UV~-rkhPuCIY5jo9fkd3olZhaxNvRwzNcB-ugfWvnk2BwA7GclBOAF8dSNvLVMd4t3~HheIH2gVVbmCihRoJsq-BkZAnHZqEHZc~r04UP0i798dEqS06xxkqOpnc9JprIV-ONr7A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Release of sCD100 from the surface of 2B4 cells stimulated with ConA.
2B4 cells (1 × 107 cells/lane) were surface-labeled with biotin and then cultured in the presence or absence of ConA (4 μg/mL) for the time indicated, at 37°C. Cell lysates (1% NP-40) or culture supernatants (sup) were immunoprecipitated with BMA-12. Immunoprecipitates were subjected to SDS-PAGE in reducing (2ME [+]) or nonreducing (2ME [−]) conditions, electrophoretically transferred to nitrocellulose membranes, and blotted with streptavidin-POD. Molecular weight markers (Mr) are indicated on the right.
