Lymphocyte proliferative response to autologous G1nBgSvNa8.1-transduced stromal cells.

Freshly harvested PBMCs were cultured for 96 hours in the presence of autologous stromal layers that had been either mock transduced with media alone or transduced with the identical vector employed for the in utero gene transfer studies. Lymphocyte proliferation was then quantitated using a commercially available BrdU proliferation kit as described in “Materials and methods.” Stimulation index is the ratio of the proliferation (BrdU uptake) of PBMCs cultured over transduced autologous stroma to the proliferation of PBMCs cultured over mock-transduced autologous stroma. Sheep nos. 7119, 7122, and 7124 are age-matched control sheep, while sheep nos. 698, 703, and 704 are in utero–transduced sheep.