Northern blot analysis of p21 expression in 32D cells with and without treatment with SCFADs.

The mRNA was prepared from cells cultured for 1 day. p21 expression was induced 3-fold with arginine butyrate (AB) compared to control (C) 0.5 U/mL IL-3 alone. In contrast, the short-chain fatty acid derivatives, 2,2 dimethylbutyric acid (1); α-methylhydrocinnamic acid (2); DL-α amino-n-butyric acid (3); phenoxyacetic acid (4); and 2,2 dimethylmethoxyacetic acid (5) were not significantly different from the control cells treated with 0.5 U/mL IL-3 alone.