Fig. 1.
Induction of γ-globin gene transcription by the SCFADs.
K562 cells stably transfected with a construct containing HS2 linked to the γ-globin gene promoter and the reporter gene EGFP were cultured with certain SCFADs for 24 hours. The total fluorescence of 2.5 × 105 cells per well was measured in a cytofluorometric plate reader and is expressed in arbitrary units including the standard deviation of each condition performed in quadruplicates. Test compounds were added at 1 mM. C, untreated control cells; AB, arginine butyrate; 1, α-methylhydrocinnamic acid; 2, 2,2 dimethyl butyric acid; 3, 3-(3,4-dimethoxyphenyl) propionic acid; 4, DLα–amino-n-butyric acid.
