FLT3 mutations (ITDs) in elderly AML patients.

(A) PCR products of the FLT3 gene after electrophoresis through a 2% agarose gel. The PCR products from a normal bone marrow (NL, left lane) and elderly AML samples 10 to 14 are demonstrated. High-molecular-weight bands in samples 11, 12, and 14 (arrows) were confirmed to have mutations by direct nucleotide sequencing. (B) SSCP analysis of PCR product from a normal bone marrow (NL, left lane) followed by elderly AML samples 45, 46, 69, 74, 85, 109, and 112. The 7 elderly AML samples had normal-appearing bands with agarose electrophoresis. Arrows point to shifted bands in patient samples 74, 109, and 112. Direct nucleotide sequencing confirmed ITDs in samples 74, 109, and 112.