Co-migration of SP and FN from the sera of patients with BM fibrosis.

Sera were immunoprecipitated with anti-SP and then developed sequentially for SP and FN in Western blot. Details of the techniques are described in “Materials and methods.” Representative blots of different categories of patients are shown. Lane 1, PV; lanes 2 and 3, ET; lanes 4 and 5, IMF; lanes 6 and 7, CML. No band for SP was observed in patients without BM fibrosis.