NK1.1 expression on spleen cells gated from the B220⁺CD19⁻ population.

Erythrocyte-depleted spleen cells were treated with Fcγ receptor blocker and labeled with rat antimouse PE-conjugated CD19 and FITC-conjugated CD45R (B220). NK1.1 expression was analyzed using biotinylated mouse antimouse NK1.1 (PK 136) mAb and streptavidin–CyChrome as a second-step reagent.