Dot plots and Ulex fluorescence of healthy individuals as related to their genotypes.

Typical results are presented for red blood cell spheres from 8 ABO genotypes as well as a Bombay individual and a control of unstained spheres. The first panel of each pair shows the 2-color dot plot; the second panel shows the FITC (Ulex) fluorescence only. The Bombay and OO samples were stained with Ulex only whereas the A samples were stained with Helix and Ulex, and the B samples with anti-B and Ulex. LFL indicates log of fluorescence. The LFL1 axis shows Ulex fluorescence and the LFL2 axis shows Helix (A) or anti-B fluorescence as appropriate.