Fig. 8.
![Fig. 8. Inhibition of the cleavage of domain D1 from domain D1D2D3 of u-PAR by BB94 in the supernatants and on the cell surface of hMVECs. / (A) The hMVECs were stimulated with or without 10−8 M phorbol ester PMA in the absence or presence of 10 μg/mL BB94 for 24, 48, or 72 hours or (B) the combination of 10 ng/mL bFGF and 10 ng/mL TNF-α in the absence or presence of 10 μg/mL BB94 for 72 hours. Supernatants were collected, and Triton X-100 cell lysates were prepared (only after 72 hours [C]). Immunoprecipitation and Western blot analysis were performed as described in “Materials and methods.” CM indicates conditioned medium.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/10/10.1182_blood.v97.10.3123/6/h81011057008.jpeg?Expires=1724216071&Signature=A7DQB0ZnlKiYtwgbE5OYU0W3iT2t5xxAFAEV5Z-18Siob77je28TeqNswHHBXRb9Sz6Jc8G7upnWEEoSVcP98ylKxV6PNptR5G9Rhfzv1N6iDE6I-P6QbUhltoVAhHXboQ9HlBPI9aDwA3RGMzyqUSv6qsLzyVXRZVcxqOFTkejKBXpahpl2V1~O-TzyaIjXjkU3gij0Vfc5OYovxoVfz7hvdSbASn6bcRvwd8ocIc4naDHs1VTymG3hwKOWty14qkGiOsDG~VsaYXya5rqRNu-SJHAmxbKic0WGzJDfnzZmW0YyWuiTgXZx830IVrPkwKYiHXEfO3SYZL8IL6U3Xw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Inhibition of the cleavage of domain D1 from domain D1D2D3 of u-PAR by BB94 in the supernatants and on the cell surface of hMVECs.
(A) The hMVECs were stimulated with or without 10−8 M phorbol ester PMA in the absence or presence of 10 μg/mL BB94 for 24, 48, or 72 hours or (B) the combination of 10 ng/mL bFGF and 10 ng/mL TNF-α in the absence or presence of 10 μg/mL BB94 for 72 hours. Supernatants were collected, and Triton X-100 cell lysates were prepared (only after 72 hours [C]). Immunoprecipitation and Western blot analysis were performed as described in “Materials and methods.” CM indicates conditioned medium.
