Increased u-PA binding to hMVECs after BB94 treatment.

The hMVECs were preincubated for 72 hours in M199 supplemented with 10% human serum with or without PMA (10⁻⁸ M) or the combination of bFGF (10 ng/mL) and TNF-α (10 ng/mL) in the presence of vehicle (0.1% DMSO) (▪) or 10 μg/mL BB94 (▨). Subsequently, the cells were cooled on ice and the specific binding of ¹²⁵I–DIP–u-PA to hMVECs was determined in triplicate wells as described in “Materials and methods.” The data represent mean ± SEM of 4 (PMA) or 5 (bFGF/TNF-α) independent experiments.