American Society of Hematology

Fig. 3.

$Fig. 3. Expression of E-NPP3 mRNA in KU-812 cells and PB basophils. / (A) Total RNA extracted from KU-812 cells (lane 1), neutrophils/eosinophils (lane 2), 97A6+ basophils (lane 3), and 97A6− mononuclear cells (lane 4) were subjected to RT-PCR analysis as described in “Study design.” Expression of E-NPP3 mRNA was observed in KU-812 cells and basophils but not in other cell fractions. (B) Phosphodiesterase-I activity of lysates from KU-812 cells and PB subpopulations (lanes are described in panel A) was determined using p-nitrophenyl thymidine-5′-l-monophosphate as a substrate. Data represent the mean of triplicate assays.$

Expression of E-NPP3 mRNA in KU-812 cells and PB basophils.

(A) Total RNA extracted from KU-812 cells (lane 1), neutrophils/eosinophils (lane 2), 97A6⁺ basophils (lane 3), and 97A6⁻ mononuclear cells (lane 4) were subjected to RT-PCR analysis as described in “Study design.” Expression of E-NPP3 mRNA was observed in KU-812 cells and basophils but not in other cell fractions. (B) Phosphodiesterase-I activity of lysates from KU-812 cells and PB subpopulations (lanes are described in panel A) was determined using p-nitrophenyl thymidine-5′-l-monophosphate as a substrate. Data represent the mean of triplicate assays.

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