ICSBP is expressed at reduced levels in FA-C cells.

(A) Immunoblot analysis demonstrates that ICSBP is expressed inducibly (50 ng/mL IFNγ for 24 hours) in wild-type MEFs (MEF11.1) (lane 2) at high levels. ICSBP expression in the FancC^−/−MEF line (MEF61) is significantly reduced compared with the wild-type cells (lane 4 vs lane 2). Constitutive expression of ICSBP is reduced in FA-C B lymphoblasts compared with normal and corrected cells (lanes 6 and 8 vs lanes 5 and 7). The same blot was stripped and reprobed with β-tubulin to demonstrate equivalent loading (lower panel). (B) EMSA of the GAS element of murine ICSBP demonstrates reduced IFNγ-stimulated binding in FancC^−/− MEFs compared with WT MEFs. Cells were stimulated with 1 ng/mL murine IFNγ for the indicated times.