Fig. 5.
Fig. 5. Elution profiles of 6-19J and L8D IgG3 mAbs on FPLC with a gel filtration column. / Twenty micrograms purified mAb was subjected to a Superdex 200 HR column (1 × 30 cm) equilibrated with 50 mM phosphate buffer (pH 7.4) containing 0.15 M NaCl, and elution was performed using the same buffer at a flow rate of 0.4 mL/min at 4°C with continuously monitored absorbance at 280 nm. (A) Solid line, L8D mAb; broken line, 6-19J mAb. (B) Mixture of L8D and 6-19J mAb. Arrows indicate elution positions of molecular weight markers: 1, human IgM (900 kd); 2, human IgG (150 kd); 3, human serum albumin (66 kd); 4, ovalbumin (45 kd).

Elution profiles of 6-19J and L8D IgG3 mAbs on FPLC with a gel filtration column.

Twenty micrograms purified mAb was subjected to a Superdex 200 HR column (1 × 30 cm) equilibrated with 50 mM phosphate buffer (pH 7.4) containing 0.15 M NaCl, and elution was performed using the same buffer at a flow rate of 0.4 mL/min at 4°C with continuously monitored absorbance at 280 nm. (A) Solid line, L8D mAb; broken line, 6-19J mAb. (B) Mixture of L8D and 6-19J mAb. Arrows indicate elution positions of molecular weight markers: 1, human IgM (900 kd); 2, human IgG (150 kd); 3, human serum albumin (66 kd); 4, ovalbumin (45 kd).

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