Polymorphisms of

MTHFR and MS genes. (A) Genomic DNA from each control subject was amplified with MTHFR677 primers digested with HinfI and was run on a 4% agarose gel. (B) Results for MTHFR1298 with MboII digestion. (C) Results for MS2756 with HaeIII digestion. DNA fragments stained with ethidium bromide are shown. Lane 1, positive control for wild type; lane 2, positive control for mutant type, except for heterozygote in panel C; lane 3, wild type; lane 4, heterozygote; lane 5, mutant.