Inhibition of PMN PI3K promotes transendothelial, transepithelial, and transmatrix migration.
Isolated human PMNs were examined for chemotaxis (A) or spontaneous migration (B) under agarose following pre-exposure of PMNs to vehicle (▪) or wortmannin (▨, 10 nM, 15 minutes, 22°C) toward indicated concentrations of fMLP. Data are derived from 4 separate experiments with results expressed as mean ± SEM distance migrated. In panels C and D, PMNs were pre-exposed to indicated concentrations of wortmannin and assessed for fMLP-stimulated (10−8 M) transmigration across confluent endothelia (▧, C) or epithelia (░, C) or across matrix-coated substrates (D). Transmigrated PMNs were quantified by determination of MPO content following termination of the assay. Data are derived from 8 to 12 monolayers in each condition from at least 3 separate experiments with results expressed as mean ± SEM number transmigrating PMNs .
