Structure and splicing of the P5′N-1 gene.

(A) Genomic structure of P5′N-1 gene. Exons are shown as boxes and introns as lines. Shaded exons represent the 5′ and 3′ untranslated regions. The first arrow shows the site of the codon 98 mutation, the second arrow shows the codon 177 mutation, and the third arrow shows the IVS9-1 mutation. (B) mRNA for 297–amino acid form of P5′N-1, including all the exons, though the initiator codon in exon 1 is inactive. (C) mRNA for 286–amino acid form of P5′N-1, with translation initiated by the last codon of exon 1 and with exon 2 spliced out. (D) mRNA resulting from IVS9-1, G→T mutation, with skipping of exon 9.