Relative IL-10 mRNA content in PBMC subpopulations.

Cells were negatively selected to yield fractions depleted of either CD3⁺ cells, CD2⁺ and CD3⁺ cells, CD4⁺ cells, CD8⁺ cells, B cells, monocytes/macrophages, or were enriched for CD4⁻CD8⁻ double-negative (DN) T cells by depleting all of the above cells. The mean (± SEM) results for 4 ALPS patients (shaded bars) are compared with 4 controls (lighter bars) consisting of 3 unrelated healthy controls and one related (family 3, 17-year-old boy; family 4, 14-year-old boy; family 14, 16 year-old girl; family 31, 16-year-old girl), Fas-normal, healthy control.