hMIP-1α stimulates osteoclast formation in human CFU-GM cultures.

Human CFU-GM–derived cells were prepared and then isolated using the MO1 monoclonal antibody. Highly purified CFU-GM cells were then cultured in the presence of varying concentrations of recombinant hMIP-1α. hMIP-1α significantly increased osteoclast-like cell formation in these cultures in a dose-dependent fashion. The results represent the mean ± SEM for quadruplicate determinations for a typical experiment. Similar results were seen in 4 independent experiments. *P < .05 compared to culture lacking MIP-1α.