Fig. 3.
Western immunoblot analysis of factor IX activation by FXIa in the presence of platelets.
(A) Factor IX (150 nM) was incubated with 1 nM wild-type FXIa or activated FXI/PKA4 in TBSA containing 2 mM CaCl2. At the indicated time points, samples were removed into sample buffer and processed as described in “Materials and methods.” (B) Reactions were run in the same manner as in panel A with the following additions: HK 50 nM, ZnCl2 (25 μM), and activated platelets (0.5 × 108/mL). The positions of zymogen (FIX) and activated (FIXaβ) factor IX are shown to the right of each blot. Time in minutes is shown across the top of each panel.
