Inhibition of HIV-1 replication by pretreatment with W peptide.

Hos/CD4/CCR5/FPRL1 (A) or Hos/CD4/CXCR4FPRL1 (B) cells were preincubated with W peptide at 37°C for 1 hour. The cells were then infected with R5 (Bal) or X4 (PNL4-3) HIV-1 at 37°C for 2 hours, and P24 production was measured after 72 hours. (C) Human monocyte–derived macrophages (R5, [Bal] ▪) or activated CD4⁺ T lymphocytes (X4, [Pnl4-3] ■) were preincubated with W peptide or chemokines (MIP-1α, MIP-1β, and RANTES at 500 ng/mL each with macrophages, or SDF-1α at 1 μg/mL for CD4⁺ T cells) at 37°C for 1 hour, followed by infection with R5 (macrophages) or X4 (CD4⁺ T cells) HIV-1 at 37°C for 2 hours. The cell culture supernatants were then measured for P24 production after 72 hours. *P < .01 compared with cells not treated with W peptide.