American Society of Hematology

Fig. 2.

$Fig. 2. Cells cultured for 7 or 14 days in AFT024 in noncontact culture with FL + SCF + Il-7 produce multilineage engraftment in NOD/SCID mice. / Six weeks after transplantation, BM was obtained from the femurs and tibiae of NOD/SCID mice and assessed for multilineage human engraftment by 3-color flow cytometry. Cells were initially gated on both the myeloid and lymphoid fractions. Isotype-matched controls are demonstrated in the top row. Cells that expressed human specific CD45 PerCP and were negative for murine specific CD45 FITC were then gated (second row). Expression of human-specific CD3 PE, CD14 PE, CD19 PE, CD33 PE, and CD34 PE was evaluated. Expanded cells expressed similar levels of each marker compared as day 0 cells (Table 3). IgG indicates immunoglobulin G.$

Cells cultured for 7 or 14 days in AFT024 in noncontact culture with FL + SCF + Il-7 produce multilineage engraftment in NOD/SCID mice.

Six weeks after transplantation, BM was obtained from the femurs and tibiae of NOD/SCID mice and assessed for multilineage human engraftment by 3-color flow cytometry. Cells were initially gated on both the myeloid and lymphoid fractions. Isotype-matched controls are demonstrated in the top row. Cells that expressed human specific CD45 PerCP and were negative for murine specific CD45 FITC were then gated (second row). Expression of human-specific CD3 PE, CD14 PE, CD19 PE, CD33 PE, and CD34 PE was evaluated. Expanded cells expressed similar levels of each marker compared as day 0 cells (Table 3). IgG indicates immunoglobulin G.

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