fMLP and T-20 peptide selectively suppress IL-12 p70 production by elutriated human monocytes.
(A) Monocytes were preincubated with varying concentrations of fMLP (●), T-20 peptide (○), or F-peptide (▴) and then were stimulated with SAC and IFN-γ. (B) fMLP and T-20 suppress both T-cell–dependent and T-cell–independent production of IL-12. Elutriated monocytes were pretreated with either fMLP (10−5 M) or T-20 peptide (10−5 M) and were stimulated with IFN-γ and either LPS (▪) or CD40L (■). (C) fMLP and T-20 selectively suppress IL-12 production. IL-10 (▨), TGF-β (▪), and TNF-α (░) production by human monocytes stimulated with IFN-γ and SAC after preincubation with either media alone, fMLP (10−5 M), or T-20 peptide (10−5 M). Cytokine concentrations were measured 24 hours after stimulation by ELISA. Data are mean ± 1 SD from monocytes obtained from 3 separate donors. (*P < .01; **P < .005).
